Many people are confused about which lactobacillus species to buy. This article discusses the differences between L. rhamnosus and L. johnsonii. Learn more about each species and what they offer for your health and well-being. Here are the pros and cons of both species. You can also try both species for a full spectrum of benefits. And don’t worry; you can even try both to see which one you prefer.
The lactic acid bacteria L. rhamnosus are frequently used in fermented dairy products and colonize the gastrointestinal tract of humans. The study aimed to characterize the carbohydrate utilization capability of human isolates and food-derived strains of L. rhamnosus. The study focused on 25 of 65 isolates of this bacterium and examined their carbohydrate utilization capacity.
The study revealed that several L. rhamnosus strains exhibit different metabolic profiles and genetic clades. High-resolution phenotyping revealed 11 distinct subgroups of strains, and the resulting genetic clades suggest niche enrichment. High-resolution carbohydrate utilization profiling identified 14 carbon sources that differed significantly among the strains. Strains were precultured on MRS medium and transferred to PM plates in duplicate.
It has been reported that various bacteria colonize a significant number of healthy individuals. However, there is some concern that L. rhamnosus is pathogenic, particularly to infants. While the bacteria are considered beneficial in many other areas of the body, they have been linked to endocarditis in some people. As a result, lactobacillus rhamnosus is still on the list of bacterium strains with qualified presumed safety status.
The researchers of ISAPP’s project have compiled an infographic to educate scientists and consumers alike about the bacterial community’s importance to the human body. The infographic features a photo of each strain and details its properties and microbial diversity. The infographics made Lactobacillus more accessible to consumers and scientists alike.
Genetic studies have revealed that the diversity of Lactobacillus species is widespread, and several strains are only loosely related. DNA analysis tools have revealed that many species were genetically related, and the current Lactobacillus genus is no longer strictly related to the existing species. In categorizing the species, scientists have proposed 25 novel genera of the Lactobacillus genus.
The Lactobacillus genus dominates the vaginal environment of healthy women. In addition to their vaginal microbiota, these bacteria can produce antimicrobial compounds. Furthermore, they are highly resistant to many antibiotics. For these reasons, L. rhamnosus is a highly beneficial lactobacillus.
The Lactobacillus rhamnose is a friendly bacteria found in the digestive tract. It is used to treat diarrhea, improve gut health, and prevent cavities. It is found in dairy products and as a probiotic supplement. When added to food, L. rhamnosus is added to cheese for flavor enhancement. Regardless, it is essential to note that many of these products don’t list L. rhamnosus as an ingredient, but it is likely to be present in the final product.
Pilus is a protein-encoding structure found in the genomes of Lactobacillus rhamnose. Pilus genes are organized into operons and encode pilin subunits and sortases. The pilin subunits are highly expressed in the GG strain, and immunogold electron microscopy demonstrated adhesion between this bacterium and human mucus. Additionally, a comparative genomic analysis with the LC705 strain provided a framework for a molecular mechanism.
The authors of this study used draft genome sequences of eight strains to identify candidate genes associated with discriminative carbohydrate utilization. The identified genes were confirmed using heterologous expression in a strain lacking the gene. This study expanded the knowledge of L. rhamnosus and its effects on human health. The study further explored the relationships between the genotype and carbohydrate utilization.
Another important effect of the lactobacillus strains is their ability to prevent urinary tract infections. Lactobacillus rhamnosus is known to reduce the risk of pneumonia in people on a breathing machine in a hospital. Lactobacillus rhamnosus GG (Culturelle) has also been shown to decrease the incidence of weight loss in women.
Several AFLP clones have been identified in L. rhamnosus based on AFLP analysis. Two strains were the type strain, HN001, while three others represent closely related species. These AFLP analyses helped in the construction of strain-specific orthologous gene matrices. Further studies are needed to determine the genetic structure of L. rhamnosus and its diversity.
L. rhamnosus is known to produce a protein called Mucus-Binding Pili. The high level of adhesion observed in the GG strain results from protein-mediated strain-specific adhesion. Comparative genomic analysis has revealed multiple types of protein in strain GG compared to L. rhamnosus strain LC705.
Genetic studies have identified several strains of Lactobacillus johnsons. These strains were isolated from the human vagina and genetically identified using amplified ribosomal DNA restriction analysis. Molecular markers of gene expression were then used to determine their relationships. The results showed that these strains belonged to distinct clusters based on the host. These clusters were derived from multiple studies involving this species.
These researchers analyzed the genome of a strain of the species and found that the environment significantly influenced the gene expression levels in the gastrointestinal tract. They also analyzed metabolites produced by the species and concluded that L. johnsonii is likely to benefit the human gastrointestinal tract. The microbial community is composed of over 500 species of bacteria, including Lactobacillus johnsonii.
Several strains of L. johnsonii have been studied in clinical trials. Interestingly, some strains inhibited the growth of the intestinal pathogen C. jejuni in vitro. These findings highlight the importance of a wide range of probiotic formulations in treating various gastrointestinal disorders. The research team will use this data to determine the potential of Lactobacillus johnsonii for clinical trials.
The optimal growth conditions for L. johnsonii CRL 1294 were 37degC and pH 6*5). The cultures were then freeze-dried in a saline solution and stored at -20degC. The final pH level was higher in the latter than in the former. These cultures were also able to auto-aggregate at 45degC.
To identify isolates containing L. johnsonii, 104 fecal samples were collected. These samples were analyzed using a terminal restriction fragment length polymorphism (T-RFLP). RFLP showed that these isolates were exclusively related to each other. In contrast, isolates from E. faecalis had high variation. Thus, the diversity index of L. johnsonii strains was approximately 12.3%.
The authors also investigated auto-aggregation. Auto-aggregation was found to be a major factor in C. jejuni infection. Nevertheless, L. johnsonii co-associated with C. jejuni infection did not affect macrophage and monocyte counts in the large intestine. The data presented in Table 3 were significant enough to determine the role of auto-aggregation in L. johnsonii growth.
Aside from preventing campylobacteriosis in humans, L. johnsonii has also been shown to reduce the production of inflammatory cytokines in laboratory mice. In a murine leukemia model, administration of L. johnsonii reduced IL-6 and MCP-1 in blood. As such, it has the potential to be used in human clinical trials as an adjuvant treatment for campylobacteriosis.
The type strain of Lactobacillus johnson is ATCC 33200. The strain was identified in 1980 and is a key part of the healthy vaginal microbiota. It was proposed as a probiotic dairy supplement in 1995. In the meantime, the LC1 strain was added to Nestle yogurt products. These cultures have shown promising results in treating several gut health problems, including H. pylori infection.
Despite its countless benefits, the bacteria may also have some side effects. In addition to improving the digestive tract, Lactobacillus can improve lipid metabolism, prevent subclinical necrotic enteritis, and enhance overall immunity in broilers. The first study involved 450 1-day-old male chicks fed a basal diet with and without BS15/g. The study was completed after 42 days.
Another study has shown that certain strains of Lactobacillus may help treat bacterial vaginosis. In Switzerland and Denmark, lactobacillus acidophilus suppositories and vaginal tablets have reduced the number of days affected by cold symptoms by up to eighteen percent. In addition, the use of Lactobacillus GG may reduce diarrhea caused by antibiotics.