Lactobacillus Acidophilus NCFM
Scientists have found that Lactobacillus acidophilus NCFM binds to B(a)P in simulated PM2.5. With environmental pollution becoming a larger problem, it is possible that probiotic bacteria can help reduce the toxicity of these contaminants. If these bacteria can be used in this manner, the world will soon be free of these environmental hazards. It will be an important step toward creating a new product that will address this problem.
In mice, the anti-inflammatory effect of Lactobacillus acidophilus NCFM is accompanied by resistance to colitis. To identify the exact mechanism responsible for this response, US researchers deleted the gene encoding phosphoglycerol transferase (PGT), a protein responsible for synthesizing the glycerol chain in lipoteichoic acid. They then induced regulatory immunity in mice and found that mice injected with L. acidophilus ncfm interleukin 10 were resistant to colitis and improved symptoms.
Several studies have demonstrated that Lactobacilli reduce the inflammatory response. Moreover, the inhibitory effect of Lactobacilli relies on Treg cells, which increase IL-10 levels and inhibit the proliferation of bystander T cells in an IL-10-dependent manner. This anti-inflammatory effect of Lactobacillus acidophilus ncfm is associated with reducing plasma cholesterol levels and ox-LDL.
The probiotic Lactobacillus acidophilus NCFM(r) is also known to enhance the immune system. It has been studied in a study of six strains of Lactobacillus acidophilus in animals. Interestingly, nine of ten lactose-intolerant subjects had reduced symptoms after ingesting lactose-inoculated milk compared to the control group.
Previously, we have shown that L. acidophilus NCFM stimulates monocyte-derived DCs. However, the LTA-deficient strain suppresses immune responses and instigates induced regulatory T cells. These effects suggest that LTA can play an important role in inhibiting inflammatory bowel disease. To further investigate these findings, future studies are needed. Here, we report the results of a study using human DCs stimulated with L. acidophilus NCFM.
The NCFM DCs contain nine Mub proteins, each with a signal peptide that can be ligated directly to DC-SIGN. This ligation process was induced by incubating the mutant with the DCs without the parent strain. The chimeric protein was removed from the cells by a chaotropic reagent.
In addition, Lactobacillus acidophilus recombinant DCs can stimulate human myeloid DCs and induce the secretion of cytokines. Compared to NCK1895 and NCK2160, IL-1b secreted more than either of those two proteins. Other DCs, such as IL-8 and IFN-a2, were barely detected in DC cultures.
Researchers have shown that L. acidophilus NCFM stimulates chemokine and cytokine production in human IEC. This bacterial strain induces a sustained response compared to E. coli Nissle, which induces a transient response. Although the study has not ruled out the possibility that L. acidophilus NCFM might be able to inhibit IEC function, it is not clear yet which factors are involved.
In animal models and clinical trials, Lactobacillus acidophilus reduces C. Albicans colonization. While the mechanisms behind this beneficial effect remain unclear, recent studies have shown that L. acidophilus increases IL-10 production in macrophages. The bacteria also influence the production of pro-inflammatory and anti-inflammatory cytokines and Candida recognition.
In addition to IL-10, L. acidophilus NCFM induces the expression of chemokines IL-1a and IL-1b. The bacterial strain is also known to stimulate the production of CCL2 and CCL20. The p38 MAPK pathway is implicated in regulating the production of these cytokines. The inflammatory response is a hallmark of intestinal pathologies.
A recent study shows that Lactobacillus acidophilus NCFM, a probiotic bacterium, induces the expression of CB and MOR, two molecules involved in pain perception. The findings suggest that the gut microbiota may play a role in regulating analgesia. If this holds, it may be possible to screen for functional bacterial strains with analgesic properties.
Researchers from the INSERM added five lactobacillus strains to the gut lining of mice and rats and measured the activity of opioid and cannabinoid receptors. This study demonstrates that lactobacillus acidophilus NCFM has opioid receptors. The findings have important implications for drug development and are warranted further research. In the meantime, the benefits of lactobacillus acidophilus are evident.
The researchers found that Lactobacillus acidophilus NCFM has opioid receptors in the brain. In mice, the bacteria also increased mRNA and protein expression in the brain. However, they failed to affect SLC26A6 expression. Furthermore, lactobacillus acidophilus increased SLC26A3 promoter activity, suggesting that this bacterium may have opioid receptors on its cell surface.
Inflammation cytokines are produced in response to gut-derived bacteria. The presence of Gram-negative Escherichia coli Nissle and Gram-positive Lactobacillus acidophilus NCFM in the gut is important for infants since these bacteria influence immune regulation and cytokine production. Lactobacillus acidophilus NCFM induces the expression of chemokines and cytokines, and it downregulates the expression of the Toll-like receptor TLR4.
This study has highlighted how L. acidophilus NCFM can induce the production of inflammatory cytokines and other proteins in the intestines. In addition, L. acidophilus NCFM can enhance the production of cytokines and other pro-inflammatory mediators via the TLR and NF-kB signaling pathways. However, further research is needed to establish the precise role of the bacterial species in this process.
The researchers used BALB/c mice, which were 10 to 12 weeks old, and weighed between 20 and 24 g. They used an intragastrical injection of L. acidophilus NCFM for seven days to determine the cytokines and chemokines produced in the mice. Mice were housed in plastic cages with a constant room temperature of 22 + 2degC and a 12h light/dark cycle. Mice were allowed to eat a standard, balanced diet and access to distilled water. L. acidophilus NCFM was infused intragastrically in mice, and the daily suspension intake was 1.0 + 0.1 ml/mouse.
The probiotics called lactobacilli have several health benefits. The good bacteria found in lactobacilli increase the immune system and boost the body’s natural response to infections. In some cases, they even reduce the severity of illness, including fever, antibiotic treatment, and missed school days. Some studies have shown that taking Lactobacillus supplements can even reduce the risk of pollen allergies and childhood eczema.
Research has shown that Lactobacillus acidophilus converts polydatin into resveratrol, a natural phytochemical that may protect against inflammation, cancer, and obesity. Other lactobacilli have also been implicated in PG metabolism. One example is Lactobacillus acidophilus ncfm, which converts daidzin to equol, a protective phenol that may help prevent breast cancer.
NCFM can grow on various plant glycosides, a group of bacterial strains. The dietary fibers found in plants are known to stimulate the growth of lactobacilli. NCFM strains that grow on plant glycosides upregulate their carbohydrate metabolism and host-bacterial interactions. This study shows that dietary fibers support the growth of Lactobacillus acidophilus in a variety of human diseases.
The expression of TLR-2 and interferon-b genes in human DCs was enhanced by L. acidophilus NCFM in a study that used human DCs stimulated with L. acidophilus NCFM and TLR-2 ligands. The resulting protein levels were measured by enzyme-linked immunosorbent assay. In addition, the lactobacilli induced IL-10 and TNF-a production in human DCs.
The immune response in Caenorhabditis elegans has been well characterized, but relatively little is known about how probiotic bacteria affect the evolutionary conserved immune responses in C. elegans. Although Lactobacillus acidophilus NCFM is not toxic to C. elegans, it cannot colonize the intestine. However, conditioning of C. elegans with Lactobacillus acidophilus NCFM decreased the burden of Enterococcus faecalis infection in the intestine of C. elegans, but preexposure to Bacillus subtilis did not have any beneficial effects.
Lactobacillus acidophilus NCFM induced higher Il-12, Il-10, and Tlr-3 levels than the other strains. Moreover, the bacterial strain X37 induced TLR-3 expression, but E. coli Nissle 1917 did not. Consequently, L. acidophilus NCFM may be an important factor in developing the immune system.